Journal: Epilepsia

Article Title: Reduced Cannabinoid 2 Receptor Activity Increases Susceptibility to Induced Seizures in Mice

doi: 10.1111/epi.16388

Figure Lengend Snippet: A. Cnr2+/− and Cnr2−/− mice exhibit significantly reduced latencies to the first MJ (A) and GTCS (B) following PTZ administration compared to WT littermates. 1-way ANOVA followed by Tukey’s post-hoc comparison, n= 8/group. C. Percent of Cnr2+/− and Cnr2−/− mutants reaching GTCS, and latency to GTCS, are significantly different from WT littermates. Mantel-Cox log-rank test. D. Cnr2−/− mice exhibit higher average Racine scores when compared to WT littermates following an 18 mA electrical stimulus. Each symbol represents one mouse. Kruskall-Wallis Test with Dunn’s multiple comparisons. WT, n=16; Cnr2+/−, n=30; Cnr2−/−, n=18. For A-D, *P < 0.05, **P < 0.01, ***P < 0.001. All error bars represent mean +/− SEM.

Article Snippet: Two CB2R knockout mice ( Cnr2 −/− ) were purchased (Jackson Laboratories, Stock No.005786) and backcrossed to the C57BL/6J background (Jackson Laboratories, Stock No. 000664) for one generation.

Techniques: Comparison

Journal: Epilepsia

Article Title: Reduced Cannabinoid 2 Receptor Activity Increases Susceptibility to Induced Seizures in Mice

doi: 10.1111/epi.16388

Figure Lengend Snippet: A. Male Cnr2−/−/RH (teal stripe) and Cnr2+/−/RH (gray stripe) mutants exhibit decreased latency to the MJ when compared to WT (black) (##P < 0.01, ###P < 0.001). Cnr2+/−/RH mutants (gray stripe) also show decreased latency to the MJ when compared to RH (black stripe) (*P < 0.05) and Cnr2+/− (gray) (**P < 0.001). B. When compared to WT, RH (black stripe) (#P < 0.05), Cnr2+/−/RH (gray stripe) (###P < 0.001), Cnr2−/− (teal) (##P < 0.01 ), and Cnr2−/−/RH (teal stripe) male mutants (##P <0.01) exhibit significantly decreased latencies to GTCS. Cnr2+/−/RH mutants (gray stripe) exhibit significantly decreased latency to the GTCS compared to Cnr2+/− mice (gray) (*P < 0.05). C. Cnr2−/− /RH female mice (teal stripe) show significantly decreased latency to the MJ when compared to WT (black) (##P < 0.01), RH (black stripe) (*P < 0.05), Cnr2+/− (gray) (***P < 0.001), and Cnr2−/−(teal) (*P < 0.05) mice. D. Cnr2−/− /RH female mice (teal stripe) exhibit decreased latency to the GTCS when compared to WT (#P < 0.05) and Cnr2+/− mutant mice (gray) (**P < 0.01). 2-way ANOVA with Tukey’s post hoc comparison, n=8–12/group. Symbols above individual bars indicate significance from WT. Error bars represent mean +/− SEM

Article Snippet: Two CB2R knockout mice ( Cnr2 −/− ) were purchased (Jackson Laboratories, Stock No.005786) and backcrossed to the C57BL/6J background (Jackson Laboratories, Stock No. 000664) for one generation.

Techniques: Mutagenesis, Comparison

Journal: Epilepsia

Article Title: Reduced Cannabinoid 2 Receptor Activity Increases Susceptibility to Induced Seizures in Mice

doi: 10.1111/epi.16388

Figure Lengend Snippet: Latency to the first MJ (A) and GTCS (B) was comparable between genotypes following flurothyl exposure. 1- way ANOVA with Tukey’s post-hoc comparison, n=8/group. C. The response of Cnr2 mutant mice to KA was similar to WT littermates. 2-way rANOVA with Bonferroni post-hoc comparison, n= 8/group. All error bars represent mean +/− SEM.

Article Snippet: Two CB2R knockout mice ( Cnr2 −/− ) were purchased (Jackson Laboratories, Stock No.005786) and backcrossed to the C57BL/6J background (Jackson Laboratories, Stock No. 000664) for one generation.

Techniques: Comparison, Mutagenesis

Journal: Epilepsia

Article Title: Reduced Cannabinoid 2 Receptor Activity Increases Susceptibility to Induced Seizures in Mice

doi: 10.1111/epi.16388

Figure Lengend Snippet: A. SR144528 significantly reduced latencies to the first MJ following PTZ administration in WT mice compared to vehicle-treated controls. B. WT mice treated with SR144528 exhibit significantly reduced latencies to the first GTCS following PTZ administration compared to vehicle-treated WT mice. SR144528 treatment did not affect latencies to the first MJ or GTCS in Cnr2−/− mutants. 2-way ANOVA with Tukey’s post-hoc comparison, n=8/group. C. WT mice treated with SR144528 exhibit significantly higher average Racine scores when compared to vehicle-treated WT mice. SR144528 treatment did not affect susceptibility to 6 Hz-induced seizures in Cnr2−/− mutants. Each symbol represents one mouse. 2-way ANOVA with Tukey’s post hoc comparison, n= 8/group. *P < 0.05, ***P < 0.001. Veh, vehicle; sr, SR144528. All error bars represent mean +/− SEM.

Article Snippet: Two CB2R knockout mice ( Cnr2 −/− ) were purchased (Jackson Laboratories, Stock No.005786) and backcrossed to the C57BL/6J background (Jackson Laboratories, Stock No. 000664) for one generation.

Techniques: Comparison

Journal: Epilepsia

Article Title: Reduced Cannabinoid 2 Receptor Activity Increases Susceptibility to Induced Seizures in Mice

doi: 10.1111/epi.16388

Figure Lengend Snippet: The CB2R agonist JWH-133 did not significantly alter latencies to the first MJ (A) or GTCS (B) in WT, Cnr2+/− or Cnr2−/− mutant mice. 2-way ANOVA with Tukey’s post-hoc comparison, n=8/group. Veh, vehicle; jwh, JWH-133. All error bars represent mean +/− SEM.

Article Snippet: Two CB2R knockout mice ( Cnr2 −/− ) were purchased (Jackson Laboratories, Stock No.005786) and backcrossed to the C57BL/6J background (Jackson Laboratories, Stock No. 000664) for one generation.

Techniques: Mutagenesis, Comparison

Journal: Epilepsia

Article Title: Reduced Cannabinoid 2 Receptor Activity Increases Susceptibility to Induced Seizures in Mice

doi: 10.1111/epi.16388

Figure Lengend Snippet: All groups of mutant male (A-B) and female (C-D) mice exhibited reduced average latencies to both the MJ and GTCS compared to WT (###P < 0.001, #P < 0.05). A. Male Cnr2−/−/RH mutants (teal stripe) show decreased latency to the MJ compared to both Cnr2+/− (gray) and RH (black stripe) (***P < 0.001). B. Both Cnr2+/−/RH (gray stripe) and Cnr2−/−/RH male mice (teal stripe) exhibit decreased latency to GTCS when compared to Cnr2+/− mice (gray) (**P < 0.01, ***P < 0.001). C. Female Cnr2+/−/RH (gray stripe) and Cnr2−/−/RH (teal stripe) mice exhibit decreased latencies to the MJ when compared to Cnr2+/− (gray) mutant mice (***P < 0.001). D. Female Cnr2+/−/RH (gray stripe) and Cnr2−/−/RH (teal stripe) mice exhibit decreased latencies to the GTCS when compared to Cnr2+/− (gray) mutant mice (***P < 0.001). RH female mice (black stripe) exhibit decreased latency GTCS when compared to Cnr2+/− (gray) mutant mice (*P < 0.05). 2-way ANOVA with Tukey’s post-hoc test, n=8–12/group. Symbols above individual bars denote statistical significance from WT. All error bars represent mean +/− SEM.

Article Snippet: Two CB2R knockout mice ( Cnr2 −/− ) were purchased (Jackson Laboratories, Stock No.005786) and backcrossed to the C57BL/6J background (Jackson Laboratories, Stock No. 000664) for one generation.

Techniques: Mutagenesis

Journal: iScience

Article Title: Yin and yang of cannabinoid CB1 receptor: CB1 deletion in immune cells causes exacerbation while deletion in non-immune cells attenuates obesity

doi: 10.1016/j.isci.2022.104994

Figure Lengend Snippet:

Article Snippet: B6.129P2-Cnr2 tm1Dgen /J mice (common name: CB2R KO; also known as CB2 −/− ) , The Jackson Laboratory , Cat# 005,786; RRID:IMSR_JAX:005,786.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Transgenic Assay, Control, Software

Journal: Scientific Reports

Article Title: Characterisation and localisation of the endocannabinoid system components in the adult human testis

doi: 10.1038/s41598-019-49177-y

Figure Lengend Snippet: Antibodies used in the study, listed in alphabetical order.

Article Snippet: CNR2 (CB2-R): Cannabinoid-binding receptor-2 , 1:400 , TEG , Rabbit , Santa Cruz Biotechnology (sc-25494).

Techniques:

Journal: Scientific Reports

Article Title: Characterisation and localisation of the endocannabinoid system components in the adult human testis

doi: 10.1038/s41598-019-49177-y

Figure Lengend Snippet: Immunohistochemical expression in human testis tissue of the two canonical EC receptors; CNR1 and CNR2. The images on the left show a general expression pattern, and a higher magnification of an area within a stippled line is shown on the right. All pictures show a low magnification image of negative controls in the right upper corner. CNR1 staining (upper images) is visible within nuclei of primary spermatocytes and in the cytoplasm of Leydig cells. CNR2 (bottom images) is localised in the cytoplasm of all types of germ cells, except early spermatocytes, and in somatic cells, especially in Leydig cells and peritubular cells. L (leptotene) and P (pachytene) indicate early and late spermatocytes, respectively.

Article Snippet: CNR2 (CB2-R): Cannabinoid-binding receptor-2 , 1:400 , TEG , Rabbit , Santa Cruz Biotechnology (sc-25494).

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Scientific Reports

Article Title: Characterisation and localisation of the endocannabinoid system components in the adult human testis

doi: 10.1038/s41598-019-49177-y

Figure Lengend Snippet: Summary of the results showing predominant expression pattern of the ECS components in specific testicular cell types, grouped into germ cells and somatic cells. Immunohistochemical (IHC) data are shown in upper lines, with the subcellular protein localisation distinguished as nuclear (N) or cellular (C). Transcriptome data from RNA-sequencing (RNA-seq) for available cell types are listed underneath in italics . Note that the RNA-seq data for spermatocytes did not distinguish any specific stages, and that the expression of the receptor RNA isoforms was studied by qPCR.

Article Snippet: CNR2 (CB2-R): Cannabinoid-binding receptor-2 , 1:400 , TEG , Rabbit , Santa Cruz Biotechnology (sc-25494).

Techniques: Expressing, Immunohistochemical staining